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Elution profiles for the HPLC analysis of the enzymatic ribosylation of DaaPur, catalyzed by: (a) E. coli PNP; (b) calf spleen PNP, 4 h reaction in the absence of phosphate; (c) calf spleen PNP, 8 h reaction in the presence of 0.5 mM phosphate. Detection was at 280 nm (solid line) and 315 nm (broken line). Peak near 10 min retention time is the substrate.
