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. 2013 Oct 11;18(10):12587–12598. doi: 10.3390/molecules181012587

Table 1.

Kinetic parameters for the enzymatic ribosylation of purine analogues with various form of PNP as catalysts, and α-D-ribose-1-phosphate as a co-substrate. Data refer to pH 6.5.

Compound Relative a kcat Km [µM] Enzyme source
2,6-diamino-8-azapurine ~0.4% 54 calf spleen
2% 65 E. coli
2,6-diaminopurine b <0.1% - calf spleen
>120% c ~7 E. coli
8-azaguanine c 21% 100 calf spleen
~4 U/mg >200 E. coli

a relative to those determined for guanine (=100%); b data from [15], at pH 7; c relative to the parameters determined for N7-methylguanosine phosphorolysis at pH 6.5 (=100).