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Reversed-phase HPLC chromatograms of T. mesophilum culture medium (upper) and a mixture of compounds 1–3 (lower). Centrifuged cultured medium was injected directly onto the column without any prior fractionation or concentration steps. The identity of bisucaberin B (1) in both samples was confirmed by co-injection. Fractions were collected every 2 min. The siderophore activity was found only in the fraction containing peak 1 in the upper chromatogram.
