Table 2.
Melanogenesis inhibitory activity and cytotoxicity in B16 mouse melanoma cells of multiflorane-type triterpenes isolated from Cucurbita maxima seeds a.
| Compound | mean ± S.D. (%) at 10 μM | mean ± S.D. (%) at 30 μM | mean ± S.D. (%) at 100 μM | |||
|---|---|---|---|---|---|---|
| Melanin content | Cell viability | Melanin content | Cell viability | Melanin content | Cell viability | |
| 1 | 94.8 ± 0.5 | 92.7 ± 2.2 | 77.1 ± 3.8 | 84.4 ± 4.0 | 73.7 ± 3.6 | 84.3 ± 5.2 |
| 2 | 106.8 ±9.3 | 106.3 ± 8.0 | 92.2 ± 5.4 | 107.1 ± 7.4 | 28.1 ± 2.3 | 69.0 ± 4.5 |
| 3 | 91.2 ± 2.2 | 107.2 ± 5.1 | 81.8 ± 4.0 | 105.6 ± 3.1 | 51.8 ± 8.0 | 95.1 ± 4.3 |
| 4 | 98.4 ± 3.2 | 110.8 ± 4.3 | 102.2 ± 11.7 | 103.0 ± 8.2 | 95.4 ± 8.4 | 101.1 ± 5.9 |
| 5 | 76.9 ± 4.0 | 99.5 ± 3.3 | 70.9 ± 0.1 | 97.7 ± 3.1 | 67.4 ± 3.6 | 99.6 ± 2.0 |
| 6 | 107.8 ± 2.6 | 91.0 ± 1.6 | 111.8 ± 7.1 | 81.8 ± 2.1 | 82.0 ± 5.1 | 74.4 ± 3.2 |
| arbutin b | 88.9 ± 2.3 | 100.0 ± 2.7 | 72.3 ± 3.1 | 94.4 ± 1.2 | 55.3 ± 1.0 | 89.9 ± 0.3 |
a Melanin content (%) and cell viability (%) were determined based on the absorbance at 450 nm, and 540 nm, respectively, by comparison with values for DMSO (100%). Each value represents the mean ± standard deviation (S.D.) of three determinations. The concentration of DMSO in the sample solution was 2 μL/mL. b Reference compound.