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. 2013 Sep 13;18(9):11299–11310. doi: 10.3390/molecules180911299

Figure 2.

Figure 2

HSCCC separartion of the crude extract (1) attractant 1; (2) attractant 2. HSCCC separation conditions: column rotary speed, 800 rpm; column temperature, 25 °C; solvent system, n-hexane-ethyl acetate-methanol-water (1.5:1:1.5:1, v/v/v/v); mobile phase, lower phase; flow rate, 2 mL/min; detection 254 nm; retention of the stationary phase, 80%; sample injected: 160 mg in 3 mL upper organic phase and 3 mL lower aqueous phase; separation mode: head-to-tail.