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. 2013 Aug 22;18(9):10132–10145. doi: 10.3390/molecules180910132

Figure 3.

Figure 3

Modulation of IDO1 mRNA and protein by COX-2 inhibitors. Leukaemia cells were stimulated with 100 ng/mL IFN-γ for 72 h, followed by the exposure to COX-2 inhibitors for 24 h. Panel A: IDO mRNA expression by leukaemia HL-60 cells as assessed with quantitative RT-PCR. Panel B: IDO protein expression by leukaemia HL-60 cells in a representative experiment out of five with similar results. Panel C: Densitometry of western blot runs; bars depict the mean and SEM from five independent experiments. Culture conditions and lane numbers for the experiments shown in panels B and C are detailed at the bottom of panel B. Panel D: COX-2 and IDO protein expression by leukaemia HL-60 cells that were stimulated with IFN-γand then treated with nimesulide for 24 h. A representative experiment out of 3 with similar results is shown.