Figure 5.

BFA induced the expression of ER stress-related genes in Colo 205 cells. Suspension and adhesion Colo 205 cells were treated with or without 0.015 μg/mL BFA for 24 h. (A) The cells were harvested and the total RNA was extracted for RT-PCR followed by 2% agarose gel electrophoresis to detect the expression of GRP78, XBP1 and CHOP. GAPDH were used as the internal control. Representative results from at least two independent experiments were shown. (B) Fold induction of GRP78, XBP1 and CHOP was determined by quantitative RT-PCR. (mean ± SD, n = 3; *, p < 0.01 compared to GAPDH).