Figure 1.

Examples of NMR-binding assay between PDZ domains and the compound cocktails. (a) Each overlaid spectrum was derived from a PDZ domain with (red) and without (black) cocktail. Upper spectra show that no signal changes were observed with mixing of the PDZ domains and a cocktail. Lower spectra show the signal changes when PDZ domains were mixed with a cocktail. (b) Summary table of binding assays using compound cocktails. The number of the plus signs indicates the degree of signal changes (+, less than 15 signals changed; ++, less than 30; +++, over 30, respectively). The minus sign indicates no signal changes. PDZ1, PDZ3, and PDZ14 were not examined.