Table 3.
The experimental data obtained for the response variables.
| Treatment | Temperature (X1) | Buffer to sample ratio (X2) | pH of buffer (X3) | Specific activity (U/mg) | Temperature stability (%) | Storage stability (%) | Surfactant agent stability (%) |
|---|---|---|---|---|---|---|---|
| 1 | −15 | 8:1 | 4.0 | 4.4 | 38.2 | 42.1 | 37.3 |
| 2 | −15 | 2:1 | 12.0 | 6.2 | 26.3 | 34.2 | 28.1 |
| 3 | 25 | 8:1 | 12.0 | 10.4 | 62.1 | 73.2 | 66.1 |
| 4 | 25 | 2:1 | 4.0 | 7.3 | 51.7 | 56.8 | 47.2 |
| 5 c | 5 | 5:1 | 8.0 | 15.3 | 78.0 | 88.1 | 82.5 |
| 6 c | 5 | 5:1 | 8.0 | 14.2 | 77.8 | 87.5 | 83.0 |
| 7 c | 5 | 5:1 | 8.0 | 15.3 | 78.0 | 88.0 | 83.0 |
| 8 | 25 | 8:1 | 4.0 | 9.2 | 58.3 | 62.1 | 54.1 |
| 9 c | 5 | 5:1 | 8.0 | 15.1 | 77.6 | 88.0 | 82.2 |
| 10 | −15 | 2:1 | 4.0 | 3.1 | 17.2 | 23.4 | 26.1 |
| 11 | −15 | 8:1 | 12.0 | 5.8 | 45.1 | 47.2 | 41.4 |
| 12 | 25 | 2:1 | 12.0 | 8.4 | 34.1 | 41.5 | 33.2 |
| 13 c | 5 | 5:1 | 8.0 | 15.2 | 78.0 | 87.9 | 83.0 |
| 14 | 5 | 9:1 | 8.0 | 12.1 | 69.2 | 70.3 | 71.2 |
| 15 | −27 | 5:1 | 8.0 | 1.1 | 10.2 | 11.1 | 14.3 |
| 16 | 37 | 5:1 | 8.0 | 2.3 | 14.0 | 21.4 | 17.2 |
| 17 | 5 | 1:1 | 8.0 | 12.3 | 67.1 | 61.1 | 62.2 |
| 18 | 5 | 5:1 | 1.4 | 8.2 | 53.1 | 50.1 | 56.5 |
| 19 | 5 | 5:1 | 11.2 | 11.2 | 66.4 | 53.2 | 49.2 |
| 20 c | 5 | 5:1 | 8.0 | 15.3 | 78.0 | 88.0 | 82.4 |
c, center point.