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Matrix of the Central Composite Design (CCD).
| Treatment | Temperature (X1) | Buffer to sample ratio (X2) | pH of buffer (X3) |
|---|---|---|---|
| 1 | −15 | 8:1 | 4.0 |
| 2 | −15 | 2:1 | 12.0 |
| 3 | 25 | 8:1 | 12.0 |
| 4 | 25 | 2:1 | 4.0 |
| 5 c | 5 | 5:1 | 8.0 |
| 6 c | 5 | 5:1 | 8.0 |
| 7 c | 5 | 5:1 | 8.0 |
| 8 | 25 | 8:1 | 4.0 |
| 9 c | 5 | 5:1 | 8.0 |
| 10 | −15 | 2:1 | 4.0 |
| 11 | −15 | 8:1 | 12.0 |
| 12 | 25 | 2:1 | 12.0 |
| 13 c | 5 | 5:1 | 8.0 |
| 14 | 5 | 9:1 | 8.0 |
| 15 | −27 | 5:1 | 8.0 |
| 16 | 37 | 5:1 | 8.0 |
| 17 | 5 | 1:1 | 8.0 |
| 18 | 5 | 5:1 | 1.4 |
| 19 | 5 | 5:1 | 11.2 |
| 20 c | 5 | 5:1 | 8.0 |
