Table 6.
Antifungal chemosensitization of 2,3-DHBA (mM) to PCS (μg/mL) tested against Candida: EUCAST-based microdilution bioassays.
| Strains | Compounds | MIC | MIC | FICI |
|---|---|---|---|---|
| alone | combined | |||
| C. albicans 90028 | 2,3-DHBA | 0.2 | 0.2 | 2.0 |
| PCS | >16 a | >16 | ||
| C. albicans CAN242 | 2,3-DHBA | 0.4 | 0.2 | 0.6 |
| PCS | >16 | 2 | ||
| C. albicans CAN276 | 2,3-DHBA | 0.2 | 0.2 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata 90030 | 2,3-DHBA | 0.2 | 0.2 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata 2001 | 2,3-DHBA | 0.2 | 0.2 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata CAN252 | 2,3-DHBA | 0.4 | 0.2 | 0.6 |
| PCS | >16 | 2 | ||
| C. krusei 6258 | 2,3-DHBA | 0.4 | 0.2 | 0.6 |
| PCS | >16 | 2 | ||
| C. krusei CAN82 | 2,3-DHBA | 0.4 | 0.2 | 0.6 |
| PCS | >16 | 2 | ||
| C. krusei CAN75 | 2,3-DHBA | 0.4 | 0.2 | 0.6 |
| PCS | >16 | 2 | ||
| Mean | 2,3-DHBA | 0.3 | 0.2 | 1.1 |
| PCS | 32.0 | 15.3 | ||
| t-test b | 2,3-DHBA | - | p < 0.01 | - |
| PCS | - | p < 0.01 | - | |
| C. albicans 90028 | 2,3-DHBA | 6.4 | 6.4 | 2.0 |
| PCS | >16 | >16 | ||
| C. albicans CAN242 | 2,3-DHBA | 3.2 | 3.2 | 2.0 |
| PCS | >16 | >16 | ||
| C. albicans CAN276 | 2,3-DHBA | >6.4 | >6.4 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata 90030 | 2,3-DHBA | 1.6 | 1.6 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata 2001 | 2,3-DHBA | 3.2 | 3.2 | 2.0 |
| PCS | >16 | >16 | ||
| C. glabrata CAN252 | 2,3-DHBA | 1.6 | 1.6 | 2.0 |
| PCS | >16 | >16 | ||
| C. krusei 6258 | 2,3-DHBA | 6.4 | 6.4 | 2.0 |
| PCS | >16 | >16 | ||
| C. krusei CAN82 | 2,3-DHBA | >6.4 | >6.4 | 2.0 |
| PCS | >16 | >16 | ||
| C. krusei CAN75 | 2,3-DHBA | >6.4 | >6.4 | 2.0 |
| PCS | >16 | >16 | ||
| Mean | 2,3-DHBA | 6.8 | 6.8 | 2.0 |
| PCS | 32.0 | 32.0 | ||
| t-test | 2,3-DHBA | - | p = 1.0 | - |
| PCS | - | N/D c | - |
a PCS was tested up to 16 μg/mL. For calculation purposes, 32 μg/mL (doubling of 16 μg/mL) was used. b Student’s t-test for paired data, mean MIC or MFC of each compound (combined, i.e., chemosensitization) vs. mean MIC or MFC of each compound (alone, i.e., no chemosensitization), was determined in nine strains (Calculation was based on [36]). c N/D, not determined (all > 16 μg/mL).