Table 1.
The effects of temperature, incubation time and growth medium on the biofilm formation of E. cloacae isolates.
| B. phenotype | Parameters number (%) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BHI a | TSB a | BHI b | TSB b | |||||||||||||
| 24 h | 48 h | 24 h | 48 h | 24 h | 48 h | 24 h | 48 h | |||||||||
| 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | 25 °C | 37 °C | |
| NA | 8(27) | 0 | 0 | 0 | 12(40) | 2(7) | 0 | 0 | 3(10) | 2(7) | 0 | 0 | 8(27) | 2(7) | 0 | 0 |
| WA | 18(60) | 10(33) | 10(33) | 5(17) | 14 (47) | 17(57) | 18(60) | 4(13) | 20(67) | 11(37) | 12(40) | 4(13) | 19(63) | 14(47) | 6(20) | 2(7) |
| MA | 4(13) | 18(60) | 16(53) | 0 | 4 (13) | 10(33) | 11(37) | 10(33) | 7(23) | 14(47) | 15(50) | 9 (30) | 3 (10) | 11(37) | 16(53) | 11(37) |
| SA | 0 | 2(7) | 4(13) | 25(83) | 0 | 1 (3) | 1 (3) | 16(53) | 0 | 3(10) | 3(10) | 17(57) | 0 | 3(10) | 8(27) | 17(57) |
| T. adh. (%) |
22 (73) |
30 (100) |
30 (100) |
30 (100) |
18 (60) |
28 (93) |
30 (100) |
30 (100) |
27 (90) |
28 (93) |
30 (100) |
30 (100) |
22 (73) |
28 (93) |
30 (100) |
30 (100) |
BHI, brain heart infusion broth; TSB, tryptic soya broth; NA, non adherent; WA, weak adherent; MA, moderate adherent; SA, strong adherent; T. dh., total adherent; B. phenotype, biofilm phenotype; BHI a, colonies from Nutrient agar and grown in BHI broth; TSB a, colonies from Nutrient agar and grown in TS broth; BHI b, inoculums from Nutrient broth and grown in BHI; TSB b, inoculums from Nutrient broth and grown in TSB.