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The effect of isoalantolactone on the expression of major apoptosis regulatory proteins. PC3 cells were exposed to 20 and 40 µM of isoalantolactone for specified time intervals. Equal amounts of lysate protein were subjected to gel electrophoresis. (A,B) Expression levels of Bax, Bcl-2, survivin, caspase-3 and PARP were monitored by western blot assay. β-actin was used as loading control. Data are representative of three independent experiments with similar results.
