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Effect of the SOCE inhibitor (2-APB) on cyclooxygenase (COX)-2 and interleukin (IL)-8 expressions in A431 cells. Cells were pretreated with SOCE inhibitor, 2-aminoethoxydipheny (2-APB), for 30 min, and then treated with 25 ng/mL of epidermal growth factor (EGF) for 3 h. Expressions of (A) COX-2 and (B) IL-8 were detected by an RT-PCR. Relative quantification in the RT-PCR of (C) COX-2 and (D) IL-8 was determined by normalizing to β-actin and a control (* p < 0.05; ** p < 0.01).
