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. 2013 Oct 14;18(10):12675–12686. doi: 10.3390/molecules181012675

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) DPV curves acquired at different concentration of glucose produced by Ab₁/AFP/Ab₂-AuNPs-glucoamylase, incubated with (a) 0, (b) 0.05, (c) 0.1, (d) 1, (e) 2, (f) 5, (g) 10, (h) 20, (i) 50, (j) 100 ng/mL AFP antigen. (B) Calibration curves for detecting AFP antigen were obtained using Ab₂-glucoamylase (a) and Ab₂-AuNPs-glucoamylase (b) as the signal tag, respectively. Inset: linear relationship between the amperometric oxidation current response and the logarithm of AFP concentrations.