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Chromatography on DlyL-Sepharose 4B. The column (0.8 × 2.7 cm) was equilibrated with 150 mM NaCl plus 5 mM CaCl2 and 5 mM MnCl2. Sample (2 mg) of ovalbumin was applied to the column. The lectin affinity support was then washed with the same buffer. Elution was carried out at 25 °C at a flow rate of 3 mL/min. Arrows demonstrate the points at which eluent (α-methyl-d-mannoside) was added. Fractions of 1.0 mL were collected.
