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Effect of BOWE on IκB-α and NF-κB p65 phosphorylation in LPS-stimulated BV-2 cells: BV-2 cells were pretreated with or without the indicated concentrations of BOWE for 4 h and then stimulated with LPS (0.1 μg/mL) for 18 h. The cellular proteins from the cells were used for the detection of phosphorylated forms of IκB-α and NF-κB p65 by western blotting. β-actin was used as a loading control.
