Table 1.
Parameters of precipitating capacities of purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia) condensed tannins (CT) with bovine serum albumin (BSA) and ribulose 1, 5-disphosphate carboxylase (Rubisco) protein.
| Proteins | Parameters * | PPC | SF | SEM ǂ | P values |
|---|---|---|---|---|---|
| BSA | a0+a | 3.0 | 3.0 | 0.01 | 0.213 |
| b | 258 | 368 | 30.0 | 0.584 | |
| c | 176 | 316 | 9.9 | <0.001 | |
| PP | 233 | 364 | 9.4 | <0.001 | |
| Rubisco | a0+a | 3.0 | 2.2 | 0.02 | <0.001 |
| b | 335 | 863 | 22.1 | <0.001 | |
| c | 50 | 195 | 13.4 | 0.002 | |
| PP | 299 | 852 | 16.4 | <0.001 |
* Parameters were obtained by fitting the amount of precipitated protein (mg) and amount of CT (µg) incubated with equation: y = a0+a/(1+exp(-(x-b)/c)), where y = mg of protein (BSA or Rubisco) precipitated , x = µg of CT incubated, a0+a = estimated maximal amount (mg) of protein (BSA or Rubisco) precipitated, b = sigmoidal centre (µg of CT at the 50% of maximal protein precipitation), and c = sigmoidal width; PP: Protein-precipitating capacity; expressed as µg CT required to precipitate 1 mg of protein (N = 12); ǂ SEM, standard error of the mean.