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Gene specific primers used for RT-PCR and real-time RT-PCR.
| Gene | Forward primer (5'–3') | Reverse primer (5'–3') |
|---|---|---|
| RT-PCR | ||
| MeCVINV1 | ATATCTAGAATGGCAGACCCCAACC | TATGTCGACAAAGATGAGTTTCACAGT |
| MeCVINV2 | ACCCTTCTTCCGTCCTTCTTC | AAATGAGGTAGACTTGGAAAGGTAAG |
| MeCVINV3 | TGAGGATCCCCAGCAAATACATGGACTAT | GCCGTCGACCAAAATAGGAGGTGTT |
| Real-time RT-PCR | ||
| MeCVINV1 | TTGAGACTAAGAGCCCGAGAAT | AGGACCAGAAGACCGAAGG |
| MeCVINV2 | CAGCCTGAGAAGAACTGGATG | GCCAGTCCATACACCATTTTG |
| MeCVINV3 | CACCTFTATTTTTCCGTCCTF | ATFCCCAACTTTCACCTTC |
