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. 2014 Sep 26;19(10):15374–15390. doi: 10.3390/molecules191015374

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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(1) The CCF is fractionated by gel filtration into four fractions (chromatogram (A) F1, F2, F3, and F4) and the induction of PAL activity is evaluated on F1 and F2 fractions. (2) The active fraction (F1) is separated by gel filtration into three subfractions (chromatogram (B) F1a, F1b and F1c). (3) F1b fraction was then deproteinized by the proteinase K. Each fraction (400 µg/slice) was tested on potato tubers for their protection effect against P. atrosepticum.