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. 2014 Sep 9;19(9):14052–14065. doi: 10.3390/molecules190914052

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The effects of DME treatment on the promastigote plasma membrane integrity (A) Overlay flow cytometry histograms of control and treated cells submitted to PI staining. A slight increase in PI fluorescence signal could be observed in cells treated with 2 × IC₅₀ of DME, whereas strong PI labeling could be observed in ethanol-treated parasites. (B) Confocal images cells treated with 1 × IC₅₀ and 2 × IC₅₀ of DME and submitted to staining with PI. Note the presence of cells with severely altered morphology not stained with PI (white arrow). DIC = Differential Interference Contrast.