Table 2.
Compounds 1, 8 and 12 treatment-induced changes in the mitochondrial membrane permeabilities for CoN, MCF-7 and PC-3 cells. The cells were stained with rhodamine 123, and then analyzed by flow cytometry. The table shows the percentage values of rhodamine 123 stained cells treated without or with compounds 1, 8 and 12 (50–100 μM) for the different cell lines (* p < 0.05 or ** p < 0.001 vs. control treated cells, assigned 100%). As positive control, FCCP (10 μM) was used.
| CoN | MCF-7 | PC-3 | ||||
|---|---|---|---|---|---|---|
| 50 µM | 100 µM | 50 µM | 100 µM | 50 µM | 100 µM | |
| Comp. 12 | 102.6 ± 9.1 | 124.9 ± 9.7 | 111.4 ± 8.9 | 111.3 ± 9.8 | 139.1 ± 12.4 | 149.3 ± 21.3 |
| Comp. 8 | 96.0 ± 7.6 | 52.3 ± 8.4 * | 93.3 ± 7.1 | 72.3 ± 6.4 * | 136.2 ± 18.5 | 92.8 ± 10.4 |
| Comp. 1 | 49.1 ± 6.6 * | 11.8 ± 2.9 ** | 77.0 ± 6.5 * | 19.4 ± 1.5 ** | 111.6 ± 8.9 | 11.9 ± 2.4 ** |
| Control | 93.2 ± 8.7 | 95.8 ± 9.9 | 98.1 ± 10.5 | |||
| FCCP | 6.8 ± 1.3 ** | 7.4 ± 0.4 ** | 5.2 ± 0.4 ** | |||