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. 2014 Jul 3;19(7):9380–9402. doi: 10.3390/molecules19079380

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Typical recovery curves obtained after FRAP experiments: the fluorescence intensity is measured before (step 1, in green), during (step 2, in red) and after photobleaching (step 3, in blue), while equilibrium is taking place. Several cases can be observed depending on the velocity and the amplitude of the recovery. Recovery can be fast with a maximal recovery (A), or slow with a maximal recovery (B). It can also be fast with a limited recovery (C) and even slow with a limited recovery (D).