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Construction of expressed vector pET32a (+) S. (a) line A, MscI digested of vector pET32a (+), the 5900 bp bands were present. Line M, DL6000 Marker; (b) line B, PCR products of linear vector pET32a (+), the 5900 bp band was present. Line M, DL6000 Marker; (c) the result of colone; (d) PCR products of pET32a (+) S, the 604 bp band was present. Line M, DL6000 Marker.
