Figure 6.

Time course of levan synthesis by the wild-type Lsc3 protein (Lsc3 wt) and fourteen mutants. Reaction was conducted at 37 °C on flat-bottom polystyrole microplates in total volume of 200 μL per well. Reaction mixture contained 600 mM of sucrose in McIllvaine’s buffer (pH 6.0) and 10 μg/mL of purified protein. Increase of turbidity was measured at 400 nm every 15 min using Infinite M200 PRO microplate reader. Mean values of four parallel measurements are shown. Standard deviation was less than 10%.