Figure 4.

Growth hormone activated more JAK2 proteins in BPC 157-treated tendon fibroblasts. Tendon fibroblasts at 50%–60% confluency were pretreated with BPC 157 at concentration of 0.5 μg/mL for one to three days. After BPC 157 pretreatment, 0.1 μg/mL growth hormone was added for another 24 h and then total JAK2 (which was not shown) and activation of JAK2 which was determined by the level of phosphorylated JAK2 were detected by Western blot analysis (A). Experiments were done in triplicate. The levels of phosphorylated JAK2 were calculated by direct densitomeric analysis of the blot (B). The “★” would be applied if there is statistically significant.