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. 2014 Apr 1;19(4):3940–3954. doi: 10.3390/molecules19043940

Figure 1.

Figure 1

Effect of WEAT on RANKL-induced osteoclast differentiation in BMMs. (A,B) BMMs were cultured with vehicle (distilled water) or WEAT in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/mL) for 4 days. (A) Cells were fixed and stained for TRAP. Scale bar represents 200 μm. (B) The number of osteoclasts (Oc) was counted. (C) The viability of BMMs was determined by Cell Counting Kit-8 assay in the presence of M-CSF and WEAT. (D) BMMs were cultured with WEAT (50 μg/mL) in the presence of M-CSF and RANKL, and WEAT was kept in the culture or withdrawn on day 4 by medium change. The number of osteoclasts was counted on day 8. (E) BMMs were cultured in the presence of M-CSF and RANKL for 4 days. WEAT was added to the medium at the indicated days, and the number of osteoclasts was counted on day 4. (F) BMMs were cultured with vehicle (dimethyl sulfoxide) or the indicated compounds in the presence of M-CSF and RANKL for 4 days, and the number of osteoclasts was counted. * p < 0.05; ** p < 0.01 versus vehicle-treated control.