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. 2014 Apr 1;19(4):3940–3954. doi: 10.3390/molecules19043940

Figure 2.

Figure 2

Effect of WEAT on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were cultured with vehicle or WEAT (50 μg/mL) in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/mL) for the indicated days. (A) mRNA expression levels of c-Fos and NFATc1 were determined by real-time quantitative polymerase chain reaction (qPCR). ** p < 0.01 versus vehicle-treated control. (B) Protein expression levels of c-Fos and NFATc1 were determined by western blot analysis. Total GAPDH was used as a loading control.