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Comparison of SSCs and 2 k particles obtained from commercial crab chitin. Microscopic analysis of (A) 2 k chitin viewed by phase contrast microscopy; and (B) super small chitin particles (SSCs) that were stained with CFW and viewed by fluorescence microscopy; (C) Flow Cytometry analysis of SSCs. Red and blue indicate the profiles of 0.2 and 2 µm standard beads, respectively; green indicates the profile of SSCs. Arrow in (A) points to a spike-shaped chitin particle. Bars, 10 µm.
