Table 4.
Effects of resveratrol on lipolysis in in vitro and in vivo studies.
| Authors | Experimental Design | Effects |
|---|---|---|
| Lasa et al. (2012) [22] | 3T3-L1 adipocytes 1, 10, 25 µM resveratrol, resveratrol-4'-O-glucuronide, resveratrol-3-O-glucuronide and resveratrol-3-O-sulfate 24 h |
↓ TG content: resveratrol, resveratrol-4'-O-glucuronide, resveratrol-3-O-glucuronide ↑ SIRT1 mRNA: resveratrol, resveratrol-4'-O-glucuronide, resveratrol-3-O-glucuronide ↑ PGC-1α mRNA: resveratrol ↑ ATGL mRNA: resveratrol ↑ HSL mRNA: resveratrol-4'-O-glucuronide |
| Szkudelska et al. (2009) [43] | Rat adipocytes 1, 10, 100 µM 90 min |
↑ Epinephrine-stimulated glycerol release (100 µM) ↑ Anti-lipolytic effect of insulin (1, 10, 100 µM) ↑ Epinephrine-stimulated glycerol release in resveratrol pre-incubated adipocytes (100 µM) |
| Gómez-Zorita et al. (2013) [45] | Obese Zucker (fa/fa) rats | ↑ HSL mRNA = ATGL and SIRT-1 mRNA |
| 15 mg/kg body weight/day (gavage) | ||
| Chow diet | ||
| 6 weeks | ||
| Alberdi et al. (2011) [47] | Male Sprague-Dawley rats | ↑ HSL mRNA = ATGL mRNA |
| High-fat, high sucrose diet | ||
| 30 mg/kg body weight/day | ||
| 6 weeks | ||
| Shan et al. (2013) [62] | Porcine adipocytes | ↑ Glycerol release at 50 μM ↑ SIRT1 and ATGL mRNA at 50 μM |
| 25 or 50 μM r | ||
| 24, 48 h | ||
| Lasa et al. (2012) [63] | 3T3-L1 adipocytes 100 µM 12, 24, 48 h |
↑ Free fatty acid release ↑Isoproterenol-stimulated glycerol release (100 µM) ↑↑ ATGL mRNA and protein expression = HSL mRNA levels |
| SGBS adipocytes | ↑ ATGL mRNA and protein expression = HSL mRNA levels |
|
| 100, 200 µM | ||
| 12, 24, h | ||
| C57BL/6 mice (WT, ATGL KO, HSL KO) | ↑ Free fatty acids release in WT and HSL KO mice = Free fatty acids release in ATGL KO mice |
|
| Epididymal fat pad adipocytes | ||
| 100 µM | ||
| 8 h | ||
| Rosenow et al. (2012) [64] | SGBS adipocytes | ↓ Intracellular TG content ↑ Glycerol release at 75 μM |
| 5, 50, 75, 100, and 200 μM | ||
| Pedersen et al. (2008) [65] | Human (obese women) abdominal subcutaneous adipose tissue fragments | ↑ Epinephrine-stimulated glycerol release |
| 50 μM | ||
| 2 h | ||
| Gómez-Zorita et al. (2013) [66] | Human (overweight women) adipocytes | ↑ Isoprenaline-stimulated glycerol release (10 μM) |
| 0.1, 10 μM | ||
| 2 h |
TG: Triacylglycerol; SIRT-1: Sirtuin 1; PGC-1α: PPAR-γ co-activator-1 α; ATGL: Adipose triglyceride lipase; HSL: Hormone sensible lipase; WT: Wild type; KO: knock-out; SGBS: Human Simpson-Golabi-Behmel Syndrome.