Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 29;19(8):11096–11130. doi: 10.3390/molecules190811096

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Removal of background and illumination shading in DHE-labeled fibroblasts. Human fibroblasts were labelled with DHE/MCD for 3 min, followed by a chase in buffer medium for 1 h at 37 °C. Bleach stacks were acquired on a UV-sensitive wide field microscope and fitted on a pixel-by-pixel basis to a mono-exponential decay function in PixBleach. The fit provides the amplitude-map (A), the time constant map (B) and the background map. The TiEm calculated including the estimated background reveals image shading and some dark pixels stemming from the camera (arrows). The TiEm without background was either calculated from the summed pixel intensities (E) or as product of amplitude and time constant (F). Insets corresponding to the box in panel (A) illustrate the correction for illumination shading in panel (E,F). Bar, 10 μm.