Figure 3.

Deguelin suppressed the migration of U-2 OS cells in vitro. U-2 OS cells (5 × 10⁴ cells/well) were incubated with 0, 10 and 15 μM of deguelin for 24 and 48 h then penetrated through to the lower surface of the filter and were stained with crystal violet and were photographed under a light microscope at 200×. Quantification of cells in the lower chambers was performed by counting cells at 200×. *** p < 0.001 significant difference between deguelin-treated groups and the untreated groups as analyzed by Student’s t test. Cells were stained with crystal violet and then were examined and photographed under a light microscope at 200× (A); The quantification of cells from each treatment in the lower chambers was counted at 200× (B).