Skip to main content
. 2014 Oct 15;19(10):16588–16608. doi: 10.3390/molecules191016588

Figure 6.

Figure 6

Deguelin affect the levels of associated proteins in migration and invasion of U-2 OS cells. U-2 OS cells were treated with deguelin (0, 10, 15 μM) for different periods of time and then cells were collected and the total protein extracts were prepared and determined as described in the Experimental Section. The levels of SOS1, GRB2, PKC and Ras (A), FAK, Rho A, ROCK-1, p-PERK and IRE-1α (B), PI3K, p-AKT(Thr308), p-AKT(Ser473), NF-κB p65, iNOS and COX-2 (C), MEKK3, MKK7, p-ERK1/2, p-JNK1/2, p-p38 and p-c-JUN (D), TIMP1, TIMP2, UPA (E), MMP-2, MMP-9, MMP-13, MMP-1 and VEGF (F) expressions were estimated by western blotting as described in the Experimental Section.