Figure 7.

Deguelin affects the NF-κB p65, Rho A and ROCK-1 expression in U-2 OS cells. Cells placed on 6-well chamber slides were treated with 10, 15 μM of deguelin for 24 h, fixed and stained using anti-NF-κB p65 (A), Rho A (B) and ROCK-1 (C) antibodies (1:200) overnight and then stained with a secondary antibody (FITC-conjugated goat anti-mouse IgG at 1:200 dilution) (green fluorescence) followed by nuclear counterstaining individually performed with PI (red fluorescence). Photomicrographs were obtained using a Leica TCS SP2 confocal spectral microscope as described in the Experimental Section.