Effects of SA, sodium nitroprusside (SNP), Nω-nitro-L-arginine (L-NNA), carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and complex treatments on the production of Sal B and NO in Salvia miltiorrhiza cell cultures. The concentrations for SA, SNP, L-NNA and cPTIO were 22 mg L−1, 0.5 mmol L−1, 2 mmol L−1 and 50 μmol L−1, respectively. SA was added to subcultured Salvia miltiorrhiza cells after 8-d culture, and in the subsequent 4 h and 2 d, both contents of NO and Sal B were determined, respectively, with three replications. Different lowercase letters represented significance at 0.05.