Figure 7.

Effects of fucoidan on levels of apoptosis-related regulators in T24 cells. Cells were treated with the indicated concentrations of fucoidan for 48 h. (A) Total RNAs were isolated and reverse-transcribed. The resulting cDNAs were then subjected to PCR with the indicated primers and the reaction products were separated in 1.0% agarose gel and visualized by EtBr staining. GAPDH was used as an internal control; (B) The cell lysates were separated with SDS-polyacrylamide gel electrophoresis, and western blotting analyses were performed using the indicated antibodies and an ECL detection system.