Figure 8.

Induction of Bid truncation, cytochrome c translocation and mitochondrial membrane hyperpolarization by fucoidan treatment in T24 cells. The cells were treated with the indicated concentrations of fucoidan for 48 h. (A) Proteins on western blots were detected with anti-Bid antibody and ECL detection; (B) Cytosolic and mitochondrial extracts were prepared, resolved by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and then probed with the anti-cytochrome c antibodies. Cytochrome oxidase IV (COX IV) and lamin B were used as internal controls for the cytosolic and mitochondrial fractions, respectively; (C) The cells were stained with JC-1 for 20 min at 37 °C. The mean JC-1 fluorescence intensity was then detected using flow cytometry. The data are expressed as the means ± SD of triplicate samples (* p < 0.05 vs. untreated control).