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Time-of-addition assay. 10 µg/mL 4a, 20 µg/mL 4i, or 40 µg/mL 4r or 4s were added to RD cells at different time periods after EV71 infection. At 12 h pi, the progeny virus yield was determined (−1–0 h: viral infection period; 0–10 h: period for virus proliferation in the cells). Values are represented as the mean ± standard deviation.
