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. 2014 Aug 5;19(8):11600–11612. doi: 10.3390/molecules190811600

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(a) In vitro stability of ⁶⁸Ga-NOTA-G₃-NGR in saline at room temperature and in human serum at 37 °C for 1, 2, and 4 h; (b) In vitro inhibition of ¹²⁵I-NGR binding to CD13 receptor by NOTA-G₃-NGR in HT-1080 cells. The IC₅₀ value of NOTA-G₃-NGR was calculated to be 74.69 ± 3.91 nM (n = 3); (c) Cell uptake of ⁶⁸Ga-NOTA-G₃-NGR in HT-1080 and HT-29 cells (n = 3, mean ± SD); (d) Cell efflux of ⁶⁸Ga-NOTA-G₃-NGR in HT-1080 and HT-29 cells (n = 3, mean ± SD).