Figure 1.

Distribution of immunolabeling (in brown) for the apical sodium‐dependent bile acid transporter (ASBT) protein (A and B) and in situ hybridization (in red) for ASBT mRNA (C and D) in the ileum. In control dogs (A), immunolabeling in the apical membrane of the enterocytes was continuous. ASBT was minimally expressed in dogs with chronic inflammatory enteropathy (CIE) (B). ASBT mRNA expression was observed in both the nucleus and cytoplasm of enterocytes of control dogs (C) and dogs with CIE (D). Scale bar is equal to 20 μm, magnification of ×400 (A and B) or 50 μm, magnification of ×400 (C and D)