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. 2014 Sep 5;19(9):13990–14003. doi: 10.3390/molecules190913990

Figure 2.

Figure 2

Stable absorbance of hemin chloride and optical detection of SLO-induced hemolysis. (A) 1 mL of RBCs ruptured by distilled water (dotted line), or RBCs hemolyzed by SLO (solid line), was mixed with 1 mL HCl (2%) in acetone to convert heme to hemin chloride. Arrow: optimal absorbance (wavelength 510 nm); (B) Serially 2-fold diluted SLO was mixed with ruptured RBCs and HCl (2%) in acetone, and absorbance at 510 nm was measured.