Figure 1.

Plate diffusion test. Antibacterial activity against E. coli DH5α demonstrated on seeded agar plates by different antibiotics is indicated by clear zones where growth inhibition has occurred. Freshly grown E. coli cells (OD₆₀₀ = 0.2, 300 µL) were embedded in 20 mL warm agar in a Petri dish (diameter 9 cm). Afterwards, holes (diameter 0.6 cm) were punched out and filled with 40 µL of antibiotic solution at the mM concentrations indicated. Pictures were taken after 24 h of incubation in the presence of the antibiotics. (A) kanamycin; (B) allicin; and (C) ampicillin.