Figure 4.

Effect of allicin on elongation of primary root growth: To investigate the effects of allicin on A. thaliana seedlings (accession Col-0), seeds were sown onto sterile filter paper which had been placed on Murashige & Skoog (MS) medium beforehand. After stratification (4 °C, 48 h), the Petri plates were cultivated at an angle approx. 20° from vertical to ensure unidirectional gravitropic root growth. For allicin exposure, the filter papers were transferred onto allicin-containing medium and 48 h after further cultivation, pictures were taken and the root lengths were measured using the software ImageJ [114]. The “boxplot” was prepared with the program PAST [115]; every dot represents the measurement of one root. The two halves of one box represent the 25 and 75 percent quartile, while the horizontal line in the middle shows the median. n = total number of measurements for the given treatment.