Figure 6.

Involvement of p38 and Akt signaling pathways in fisetin-stimulated HO-1 up-regulation in BV-2 microglia. (A) Cells were pretreated with various inhibitors (PD98059, SB203580, SP600125 or LY294002) for 60 min, followed by stimulation with fisetin for 24 h. Cells were pretreated with various concentrations of SB203580 (B) or LY294002 (C) for 60 min, followed by stimulation with fisetin for 24 h. Whole cell lysis proteins was extracted and subjected to western blot analysis for HO-1 expression. Cells were incubated with fisetin for indicated periods (5, 10, 30, 60 or 120 min). Whole cell lysates were subjected to western blot analysis by using antibodies against the phosphorylated p38 (D) or Akt (E). Similar results were obtained from at least three independent experiments. * p < 0.05 compared with the control group; ^# p < 0.05 compared with the fisetin treatment group.