Table 4.
In vitro growth inhibition of selected tumor cell lines by new benzimidazole-4,7-dione derivatives.
| Compound | IC50 [μM] A549 | Differential cytotoxicity O/H | |
|---|---|---|---|
| Normoxia (O) | Hypoxia (H) | ||
| 5a | 30.2 ± 1.2 | 36.1 ± 1.2 | 0.83 |
| 5b | 100.0 ± 1.8 | 47.4 ± 1.1 | 2.13 |
| 5c | 80.9 ± 1.9 | 51.2±1.5 | 1.58 |
| 5d | 479.5 ± 3.6 | 232.4 ± 1.4 | 2.06 |
| 6a | 115.7 ± 1.9 | 35.0 ± 1.6 | 3.28 |
| 6b | 500.6 ± 1.2 | 116.0 ± 0.8 | 4.31 |
| 6c | 79.5 ± 1.9 | 44.0 ± 2.5 | 1.80 |
| 6d | 252 ± 2.5 | 96.8 ± 1.9 | 2.62 |
| Tirapazamine | 166.2 ± 1.6 | 30.0 ± 1.5 | 4.61 |
WST-1 assay was used to determine the inhibition of the cell growth after 48 h incubation with the tested compounds. IC50 values (concentration of the tested compounds causing 50% inhibition of the cell growth compared to control cells) were calculated and expressed as mean ± SD, n = 3.