Figure 6.

Effects of DMTU, BCNU and GDP on HCY2-induced protection against menadione toxicity in H9c2 cells. Cells were pre-incubated with HCY2 (25 µg/mL) in the absence or presence of DMTU (20 mM), BCNU (50 µM) or GDP (500 µM) for 4 h, followed by another 4 h incubation with menadione (12.5 µM). (a) The percentage of lactate dehydrogenase (LDH) release was measured as described in the Experimental Section; and (b) The degree of protection against menadione-induced cytotoxicity was estimated as described in the Experimental Section.

(a)* Significantly different from the respective non-menadione control group; # significantly different from the respective manadione control group; † significantly different from the respective HCY2 pre-incubated menadione group (p < 0.05); (b) * Significantly different from the HCY2 pre-incubated group (p < 0.05). Values given are means ± SD, with n = 3.