Figure 6.

Sch B induces G0/G1 phase arrest and regulates the expression of cell cycle-related proteins of gallbladder cancer cells. (A) GBC-SD and NOZ cells were treated with Sch B (0, 30, 60 and 90 μmol/L) for 48 h. The cell cycle phases of the treated cells were evaluated by flow cytometry; (B) Data were expressed as mean ± SD (n = 3); (C) The expression levels of cyclin D1, CDK4 were measured by western blot analysis, and GAPDH was used as a loading control; Results were representative of 3 independent experiments. * P < 0.05, ** P < 0.01 vs. the control group.