Table 6.

Radioligand binding assay conditions.

Receptor	Radioligand	Blank(nonspecific)	Buffer	Incubation Conditions
GABAA	[3H]muscimol	100 µM GABA	50 mM Tris-HCl pH 7.4	10 min, 0–4 °C
BDZ	[3H]flunitrazepam	10 µM diazepam	50 mM Tris-HCl pH 7.4	20 min, 0–4 °C