Table 3.
Effects of addition and incubation time of DM-301 resin on mycelial growth and production of botrallin and TMC-264 of Hyalodendriella sp. Ponipodef12 in liquid culture.
| Resin Addition Time (day) | Incubation Time/Harvest Time (day/day) | Mycelia Biomass (g dw/L) | Botrallin Yield (mg/L) | TMC-264 Yield (mg/L) | Yield of Botrallin Plus TMC-264 (mg/L) |
|---|---|---|---|---|---|
| CK | 0/20 | 6.20 ± 0.05 e | 7.26 ± 0.08 l | 3.88 ± 0.05 k | 11.14 ± 0.04 m |
| 0/22 | 6.87 ± 0.04 c | 17.17 ± 0.06 k | 4.85 ± 0.02 j | 22.02 ± 0.07 k | |
| 0/24 | 7.24 ± 0.01 a | 21.19 ± 0.02 j | 5.08 ± 0.07 j | 26.26 ± 0.05 i | |
| 0/26 | 7.35 ± 0.01 a | 22.97 ± 0.38 j | 5.61 ± 0.06 j | 28.58 ± 0.43 h | |
| 0/28 | 7.39 ± 0.01 a | 22.49 ± 0.24 i | 5.00 ± 0.18 j | 27.48 ± 0.14 hi | |
| 0/30 | 7.34 ± 0.01 a | 20.21 ± 0.03 j | 4.06 ± 0.07 k | 24.27 ± 0.07 j | |
| 0/32 | 6.93 ± 0.07 bc | 19.37 ± 0.17 j | 3.39 ± 0.03 kl | 22.76 ± 0.15 k | |
| 0/34 | 6.87 ± 0.19 c | 17.43 ± 0.82 k | 3.14 ± 0.23 l | 20.57 ± 0.78 l | |
| 0/36 | 5.69 ± 0.13 e | 17.06 ± 0.29 k | 2.81 ± 0.07 m | 19.87 ± 0.36 l | |
| 20 | 2/22 | 6.64 ± 0.11 d | 33.46 ± 0.99 g | 13.86 ± 0.24 i | 47.32 ± 1.17 g |
| 4/24 | 7.18 ± 0.15 b | 36.07 ± 0.67 f | 33.44 ± 0.11 e | 69.51 ± 0.78 e | |
| 6/26 | 6.93 ± 0.02 bc | 41.15 ± 0.36 de | 37.06 ± 0.58 c | 78.21 ± 0.84 c | |
| 8/28 | 6.91 ± 0.05 bc | 42.74±0.63 cd | 34.79±0.24 de | 77.53±0.86 c | |
| 10/30 | 6.92 ± 0.05 bc | 42.48 ± 0.36 cd | 35.08 ± 0.77 d | 77.56 ± 1.10 c | |
| 22 | 2/24 | 7.38 ± 0.02 a | 45.83 ± 0.43 c | 22.87 ± 1.10 g | 68.70 ± 1.12 e |
| 4/26 | 7.35 ± 0.24 a | 50.66 ± 0.36 a | 37.58 ± 0.07 c | 88.24 ± 0.30 b | |
| 6/28 | 7.34 ± 0.02 a | 49.86 ± 0.11 a | 37.24 ± 0.06 c | 87.11 ± 0.16 b | |
| 8/30 | 7.32 ± 0.01 a | 49.95 ± 0.30 a | 37.43 ± 0.10 c | 87.38 ± 0.36 b | |
| 10/32 | 6.98 ± 0.06 bc | 49.24 ± 0.38 ab | 36.77 ± 0.11 cd | 86.01 ± 0.42 b | |
| 24 | 2/26 | 7.34 ± 0.03 a | 40.82 ± 0.26 e | 27.69 ± 0.22 f | 68.50 ± 0.46 e |
| 4/28 | 7.55 ± 0.06 a | 51.47 ± 0.37 a | 45.85 ± 0.14 b | 97.32 ± 0.49 a | |
| 6/30 | 7.33 ± 0.04 a | 49.44 ± 0.10 ab | 47.74 ± 0.46 a | 97.18 ± 0.56 a | |
| 8/32 | 6.88 ± 0.06 c | 48.80 ± 0.47 b | 47.10 ± 0.08 a | 95.89 ± 0.48 a | |
| 10/34 | 6.85 ± 0.07 c | 48.88 ± 0.40 b | 47.07 ± 0.03 a | 95.95 ± 0.38 a | |
| 26 | 2/28 | 7.36 ± 0.04 a | 28.03 ± 1.37 i | 18.01 ± 0.12 h | 46.04 ± 1.43 g |
| 4/30 | 7.15 ± 0.05 b | 31.76 ± 0.83 h | 27.04 ± 0.19 f | 58.79 ± 0.95 f | |
| 6/32 | 6.88 ± 0.02 c | 37.27 ± 0.05 f | 36.58 ± 0.17 cd | 73.85 ± 0.12 d | |
| 8/34 | 6.78 ± 0.05 cd | 43.49 ± 0.15 c | 35.48 ± 0.51 d | 78.97 ± 0.65 c | |
| 10/36 | 5.21 ± 0.03 f | 43.68 ± 0.01 c | 35.18 ± 0.50 d | 78.85 ± 0.50 c |
Notes: CK, without addition of the test resin; the concentration of resin DM-301 in medium was 4.38% (g/mL); the values are expressed as means ± standard deviations (n = 3). Different letters (i.e., a, b, c, …) indicate significant differences among the treatments including addition and incubation time in each column at p = 0.05 level.