Table 11.

In vitro effects of glycosides and aglycones on the growth of Leishmania amazonensis in culture. Parasites were counted with a hemocytometer at 24, 48 and 72 h after adding compounds to a culture with an initial concentration of 1 × 10⁶ parasites/ml. Results shown are the mean and SEM of 3 biological replicates. Controls received only PBS

Time of exposure (h)	Control (× 106)	Amygdalin (× 106)	Esculin (× 106)	Mandelonitrile (× 106)
24	6 ± 1	8 ± 2	9 ± 2	1.1 ± 0.5*
48	26 ± 3	32 ± 4	30 ± 6	0.2 ± 0.1**
72	58 ± 5	74 ± 9	62 ± 3	0.6 ± 0***

^*P < 0.05, ^**P < 0.001, ^***P < 0.0001. Ordinary one-way ANOVA; Unpaired t-test for post-hoc multiple comparisons